Introduction: The prevalence of nonhealing wounds is predicted to increase due to the growing aging population.\r\nDespite the use of novel skin substitutes and wound dressings, poorly vascularized wound niches impair wound\r\nrepair. Mesenchymal stem cells (MSCs) have been reported to provide paracrine signals to promote wound healing,\r\nbut the effect of human Whartonâ��s jelly-derived MSCs (WJ-MSCs) has not yet been described in human normal skin.\r\nThe aim of this study is to examine the effects of human WJ-MSC paracrine signaling on normal skin fibroblasts\r\nin vitro, and in an in vivo preclinical model.\r\nMethods: Human WJ-MSCs and normal skin fibroblasts were isolated from donated umbilical cords and normal\r\nadult human skin. Fibroblasts were treated with WJ-MSC-conditioned medium (WJ-MSC-CM) or nonconditioned\r\nmedium.\r\nResults: Expression of genes involved in re-epithelialization (transforming growth factor-�Ÿ2), neovascularization\r\n(hypoxia-inducible factor-1a) and fibroproliferation (plasminogen activator inhibitor-1) was upregulated in WJ-MSCCM-\r\ntreated fibroblasts (P = 0.05). WJ-MSC-CM enhanced normal skin fibroblast proliferation (P = 0.001) and migration\r\n(P = 0.05), and promoted wound healing in an excisional full-thickness skin murine model.\r\nConclusions: Under our experimental conditions, WJ-MSCs enhanced skin wound healing in an in vivo mouse model.
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